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《医学前沿(英文)》 2012年 第6卷 第1期 页码 41-47 doi: 10.1007/s11684-012-0175-9
Umbilical cord mesenchymal stem cells (MSCs) are a unique, accessible, and non-controversial source of early stem cells that can be readily manipulated. As the most common pluripotent cell, bone marrow-derived MSCs display limitations with the progress of stem cell therapy. By contrast, umbilical cord-derived cells, which have plentiful resources, are more accessible. However, several uncertain aspects, such as the effect of donor selection or culture conditions, long-term therapeutic effects, product consistency, and potential tumorigenicity, are the bottleneck in this clinical therapy. MSCs are predicted to undergo an unprecedented development in clinical treatment when a generally acknowledged criterion emerges. In the current paper, we highlight the application of umbilical cord-derived MSCs in skin therapies based on our previous studies, as well as the achievements of our peers in this field. This paper focuses on the strategies, challenges, and potential of this novel therapy.
关键词: umbilical cord mesenchymal stem cells cutaneous regeneration
Mesenchymal stem cells hold promise for regenerative medicine
Shihua Wang, Xuebin Qu, Robert Chunhua Zhao
《医学前沿(英文)》 2011年 第5卷 第4期 页码 372-378 doi: 10.1007/s11684-011-0164-4
关键词: mesenchymal stem cells differentiation immunomodulation regenerative medicine
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《医学前沿(英文)》 2015年 第9卷 第1期 页码 20-29 doi: 10.1007/s11684-014-0371-x
Ischemic stroke is a focal cerebral insult that often leads to many adverse neurological complications severely affecting the quality of life. The prevalence of stroke is increasing throughout the world, while the efficacy of current pharmacological therapies remains unclear. As a neuroregenerative therapy, the implantation of human umbilical cord mesenchymal stem cells (hUC-MSCs) has shown great possibility to restore function after stroke. This review article provides an update role of hUC-MSCs implantation in the treatment of ischemic stroke. With the unique “immunosuppressive and immunoprivilege” property, hUC-MSCs are advised to be an important candidate for allogeneic cell treatment. Nevertheless, most of the treatments are still at primary stage and not clinically feasible at the current time. Several uncertain problems, such as culture conditions, allograft rejection, and potential tumorigenicity, are the choke points in this cellular therapy. More preclinical researches and clinical studies are needed before hUC-MSCs implantation can be used as a routinely applied clinical therapy.
关键词: cellular therapy transplantation human umbilical cord mesenchymal stem cells ischemic stroke
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《医学前沿(英文)》 2013年 第7卷 第3期 页码 345-353 doi: 10.1007/s11684-013-0282-2
Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) possess various advantageous properties, including self-renewal, extended proliferation potential, multi-lineage differentiation potential and capacity for differentiating into sweat gland-like cells in certain conditions. However, little is known about the effect of clinical-grade culture conditions on these properties and on the differentiative potential of hUC-MSCs. In this study, we sought to investigate the properties of hUC-MSCs expanded with animal serum free culture media (ASFCM) in order to determine their potential for differentiation into sweat gland-like cells. We found that primary cultures of hUC-MSCs could be established with ASFCM. Moreover, cells cultured in ASFCM showed vigorous proliferation comparable to those of cells grown in classical culture conditions containing fetal bovine serum (FBS). Morphology of hUC-MSCs cultured in ASFCM was comparable to those of cells grown under classical culture conditions, and hUC-MSCs grown in both of the two culture conditions tested showed the typical antigen profile of MSCs—positive for CD29, CD44, CD90, and CD105, and negative for CD34 and CD45, as expected. Chromosomal aberration assay revealed that the cells were stable after long-term culture under both culture conditions. Like normal cultured MSCs, hUC-MSCs induced under ASFCM conditions exhibited expression of the same markers (CEA, CK14 and CK19) and developmental genes (EDA and EDAR) that are characteristic of normal sweat gland cells. Taken together, our findings indicate that the classical culture medium used to differentiate hUC-MSCs into sweat gland-like cells can be replaced safely by ASFCM for clinical purposes.
关键词: umbilical cord mesenchymal stem cells sweat gland preclinical
Transplantation of placenta-derived mesenchymal stem cells in type 2 diabetes: a pilot study
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《医学前沿(英文)》 2011年 第5卷 第1期 页码 94-100 doi: 10.1007/s11684-011-0116-z
Mesenchymal stem cells (MSC) have been used in clinical trials for severe diabetes, a chronic disease with high morbidity and mortality. Bone marrow is the traditional source of human MSC, but human term placenta appears to be an alternative and more readily available source. Here, the therapeutic effect of human placenta-derived MSC (PD-MSC) was studied in type 2 diabetes patients with longer duration, islet cell dysfunction, high insulin doses as well as poor glycemic control in order to evaluate the safety, efficacy and feasibility of PD-MSC treatment in type 2 diabetes (T2D). Ten patients with T2D received three intravenous infusions of PDSC, with one month interval of infusion. The total number of PDSC for each patient was (1.22–1.51) × 106/kg, with an average of 1.35 × 106/kg. All of the patients were followed up after therapy for at least 3 months. A daily mean dose of insulin used in 10 patients was decreased from 63.7?±?18.7 to 34.7?±?13.4 IU (P<0.01), and the C-peptide level was increased from 4.1?±?3.7 ng/mL to 5.6?±?3.8 ng/mL (P<0.05) respectively after therapy. In 4 of 10 responders their insulin doses reduced more than 50% after infusion. The mean levels of insulin and C-peptide at each time point in a total of 10 patients was higher after treatment (P<0.05). No fever, chills, liver damage and other side effects were reported. The renal function and cardiac function were improved after infusion. The results obtained from this pilot clinical trial indicate that transplantation of PD-MSC represents a simple, safe and effective therapeutic approach for T2D patients with islet cell dysfunction. Further large-scale, randomized and well-controlled clinical studies will be required to substantiate these observations.
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《医学前沿(英文)》 2011年 第5卷 第1期 页码 33-39 doi: 10.1007/s11684-011-0114-1
Adult stem cells hold great promise for wound healing and tissue regeneration. Mesenchymal stem cells (MSCs), for example, have been shown to play a role in tissue repair. Research has shown that endogenous bone marrow MSCs or exogenously delivered MSCs migrate to the sites of injury and participate in the repair process. The precise mechanisms underlying migration of MSCs into the injured tissue are still not fully understood, although multiple signaling pathways and molecules were reported, including both chemoattractive factors and endogenous electric fields at wounds. This review will briefly summarize the regulatory facors and signaling transduction pathways involved in migration of MSCs. A better understanding of the molecular mechanisms involved in the migration of MSCs will help us to develop new stem cell-based therapeutic strategies in regenerative medicine.
关键词: mesenchymal stem cells migration molecular mechanisms signaling pathway
《医学前沿(英文)》 2022年 第16卷 第6期 页码 827-858 doi: 10.1007/s11684-022-0948-8
关键词: infertility stem cell therapy mesenchymal stem cells pluripotent stem cells
《医学前沿(英文)》 doi: 10.1007/s11684-023-0999-5
关键词: thyroid carcinoma mesenchymal stem cell extracellular vesicle GTF2I FAT1 CDK4
Mesenchymal stem cells and immune disorders: from basic science to clinical transition
Shihua Wang, Rongjia Zhu, Hongling Li, Jing Li, Qin Han, Robert Chunhua Zhao
《医学前沿(英文)》 2019年 第13卷 第2期 页码 138-151 doi: 10.1007/s11684-018-0627-y
关键词: mesenchymal stem cell clinical transition immune disorders
Immunohistochemical characterization of hepatic stem cell-related cells in developing human liver
XU Jun, HU Yong, WANG Jian, ZHANG Taiping, ZHOU Ji, YU Hongyu
《医学前沿(英文)》 2007年 第1卷 第3期 页码 264-268 doi: 10.1007/s11684-007-0050-2
关键词: origin Little mesenchymal tissue cords/mesenchymal tissue CD34
Clinical study using mesenchymal stem cells for the treatment of patients with severe COVID-19
Lingling Tang, Yingan Jiang, Mengfei Zhu, Lijun Chen, Xiaoyang Zhou, Chenliang Zhou, Peng Ye, Xiaobei Chen, Baohong Wang, Zhenyu Xu, Qiang Zhang, Xiaowei Xu, Hainv Gao, Xiaojun Wu, Dong Li, Wanli Jiang, Jingjing Qu, Charlie Xiang, Lanjuan Li
《医学前沿(英文)》 2020年 第14卷 第5期 页码 664-673 doi: 10.1007/s11684-020-0810-9
关键词: coronavirus disease 2019 (COVID-19) mesenchymal stem cell acute respiratory distress syndrome stem cell therapeutics
张荣利,冯 雪,张会亮,罗国安,李连达,王义明
《中国工程科学》 2007年 第9卷 第7期 页码 89-94
采用密度梯度离心法分离骨髓间质干细胞,差速贴壁法进行纯化,应用流式细胞仪、激光共聚焦显微镜等对纯化细胞进行鉴定,结果细胞表面抗原CD29,CD44,CD105,CD166表达呈阳性,而CD14,CD34,CD45表达呈阴性。采用RT唱PCR鉴定了三个基因:nestin,NST,Oct-4,前两者呈阳性表达,后者弱阳性表达。这些细胞特异抗原与基因表达综合起来,表明得到的细胞具备骨髓间质干细胞的特性。密度梯度分离与差速贴壁相结合,可获得较好均一性的骨髓间质干细胞,是一种简单可靠、易于推广的骨髓干细胞获取方法,可为细胞与组织工程研究提供种子细胞。
Epithelial-to-mesenchymal transition in cancer: complexity and opportunities
Yun Zhang, Robert A. Weinberg
《医学前沿(英文)》 2018年 第12卷 第4期 页码 361-373 doi: 10.1007/s11684-018-0656-6
The cell-biological program termed the epithelial-to-mesenchymal transition (EMT) plays an important role in both development and cancer progression. Depending on the contextual signals and intracellular gene circuits of a particular cell, this program can drive fully epithelial cells to enter into a series of phenotypic states arrayed along the epithelial-mesenchymal phenotypic axis. These cell states display distinctive cellular characteristics, including stemness, invasiveness, drug-resistance and the ability to form metastases at distant organs, and thereby contribute to cancer metastasis and relapse. Currently we still lack a coherent overview of the molecular and biochemical mechanisms inducing cells to enter various states along the epithelial-mesenchymal phenotypic spectrum. An improved understanding of the dynamic and plastic nature of the EMT program has the potential to yield novel therapies targeting this cellular program that may aid in the management of high-grade malignancies.
关键词: epithelial-to-mesenchymal transition cancer metastasis cancer stem cell
Determination of telomerase activity in stem cells and non-stem cells of breast cancer
LI Zhi, HE Yanli, ZHANG Jiahua, ZHANG Jinghui, HUANG Tao
《医学前沿(英文)》 2007年 第1卷 第3期 页码 294-298 doi: 10.1007/s11684-007-0056-9
间充质干细胞——新冠病毒肺炎相关急性呼吸窘迫综合征潜在的治疗选择
赵璇, 张毅
《工程(英文)》 2020年 第6卷 第10期 页码 1073-1075 doi: 10.1016/j.eng.2020.05.015
标题 作者 时间 类型 操作
Umbilical cord-derived mesenchymal stem cells: strategies, challenges, and potential for cutaneous regeneration
null
期刊论文
Mesenchymal stem cells hold promise for regenerative medicine
Shihua Wang, Xuebin Qu, Robert Chunhua Zhao
期刊论文
Implantation of human umbilical cord mesenchymal stem cells for ischemic stroke: perspectives and challenges
null
期刊论文
Capacity of human umbilical cord-derived mesenchymal stem cells to differentiate into sweat gland-likecells: a preclinical study
null
期刊论文
Transplantation of placenta-derived mesenchymal stem cells in type 2 diabetes: a pilot study
null
期刊论文
Regulatory factors of mesenchymal stem cell migration into injured tissues and their signal transduction
null
期刊论文
Cell therapy for the treatment of reproductive diseases and infertility: an overview from the mechanism to the clinic alongside diagnostic methods
期刊论文
Extracellular vesicle-carried GTF2I from mesenchymal stem cells promotes the expression of tumor-suppressive
期刊论文
Mesenchymal stem cells and immune disorders: from basic science to clinical transition
Shihua Wang, Rongjia Zhu, Hongling Li, Jing Li, Qin Han, Robert Chunhua Zhao
期刊论文
Immunohistochemical characterization of hepatic stem cell-related cells in developing human liver
XU Jun, HU Yong, WANG Jian, ZHANG Taiping, ZHOU Ji, YU Hongyu
期刊论文
Clinical study using mesenchymal stem cells for the treatment of patients with severe COVID-19
Lingling Tang, Yingan Jiang, Mengfei Zhu, Lijun Chen, Xiaoyang Zhou, Chenliang Zhou, Peng Ye, Xiaobei Chen, Baohong Wang, Zhenyu Xu, Qiang Zhang, Xiaowei Xu, Hainv Gao, Xiaojun Wu, Dong Li, Wanli Jiang, Jingjing Qu, Charlie Xiang, Lanjuan Li
期刊论文
Epithelial-to-mesenchymal transition in cancer: complexity and opportunities
Yun Zhang, Robert A. Weinberg
期刊论文
Determination of telomerase activity in stem cells and non-stem cells of breast cancer
LI Zhi, HE Yanli, ZHANG Jiahua, ZHANG Jinghui, HUANG Tao
期刊论文